Publications

The following are recent scientific publications from our lab over the past 10 years.

Qian P, Fu Y, Zeng J, Naldrett MJ, Zhang R, Sibley LD. Evolutionarily convergent mechanism of formin regulation coordinates actin polymerization in apicomplexan parasites. Sci Adv. 2025 Dec 12;11(50):eaea2136. doi:10.1126/sciadv.aea2136. Epub 2025 Dec 10. PMID: 41370370; PMCID: PMC12693976.

Zeng J, Fu Y, Qian P, Huang W, Niu Q, Beatty WL, Brown A, Sibley LD, Zhang R. Atomic models of the Toxoplasma cell invasion machinery. Nat Struct Mol Biol. 2025 Dec 9. doi: 10.1038/s41594-025-01728-w. Epub ahead of print. PMID: 41366525.

Saraav I, Huang W, Jung J, Xu R, Fu Y, McDonald K, Schriefer LA, Rodgers R, Baldridge MT, Newberry RD, Hsieh CS, Sibley LD. Early life infection with Cryptosporidium parvum induces inflammatory responses to dietary antigens. Gut Microbes. 2025 Dec;17(1):2551115. doi:10.1080/19490976.2025.2551115. Epub 2025 Aug 28. PMID: 40874311; PMCID: PMC12396125.

Huang W, Xu R, Kimball A, Witola WH, Baldridge MT, Feng Y, Xiao L, Sibley LD. Cryptosporidium parvum multidrug resistance protein confers resistance to toxic gut microbial metabolite. Cell Host Microbe. 2025 Sep 10;33(9):1589-1605.e9. doi: 10.1016/j.chom.2025.07.024. Epub 2025 Aug 27. PMID: 40876448; PMCID: PMC12477738.

Uddin T, Xie H, Mittal P, Ence CC, Patil S, Sharma A, Melillo B, Chatterjee AK, Sibley LD. Improved Bicyclic Pyrrolidine Analogues Inhibit Toxoplasma gondii Growth In Vitro and Cure Infection In Vivo. J Med Chem. 2025 Aug 28;68(16):17350-17363. doi: 10.1021/acs.jmedchem.5c00865. Epub 2025 Aug 8. PMID: 40778727; PMCID: PMC12415985.

Xu R, Beatty WL, Greigert V, Witola WH, Sibley LD. Multiple pathways for glucose phosphate transport and utilization support growth of Cryptosporidium parvum. Nat Commun. 2024 Jan 9;15(1):380. doi: 10.1038/s41467-024-44696-3. PMID: 38191884; PMCID: PMC10774378.

Greigert V, Saraav I, Son J, Zhu Y, Dayao D, Antia A, Tzipori S, Witola WH, Stappenbeck TS, Ding S, Sibley LD. Cryptosporidium infection of human small intestinal epithelial cells induces type III interferon and impairs infectivity of Rotavirus. Gut Microbes. 2024 Jan-Dec;16(1):2297897. doi: 10.1080/19490976.2023.2297897. Epub 2024 Jan 8. PMID: 38189373; PMCID: PMC10793699.

Kimball A, Funkhouser-Jones L, Huang W, Xu R, Witola WH, Sibley LD. Mendelian segregation and high recombination rates facilitate genetic analyses in Cryptosporidium parvum. PLoS Genet. 2024 Jun 17;20(6):e1011162. doi: 10.1371/journal.pgen.1011162. PMID: 38885280; PMCID: PMC11213348.

Matta SK, Kohio HP, Chandra P, Brown A, Doench JG, Philips JA, Ding S, Sibley LD. Genome-wide and targeted CRISPR screens identify RNF213 as a mediator of interferon gamma-dependent pathogen restriction in human cells. Proc Natl Acad Sci U S A. 2024 Jan 2;121(1):e2315865120. doi: 10.1073/pnas.2315865120. Epub 2023 Dec 26. PMID: 38147552; PMCID: PMC10769850.

Funkhouser-Jones LJ, Xu R, Wilke G, Fu Y, Schriefer LA, Makimaa H, Rodgers R, Kennedy EA, VanDussen KL, Stappenbeck TS, Baldridge MT, Sibley LD. Microbiota-produced indole metabolites disrupt mitochondrial function and inhibit Cryptosporidium parvum growth. Cell Rep. 2023 Jun 28;42(7):112680. doi: 10.1016/j.celrep.2023.112680. Online ahead of print. PMID: 37384526.

Huang Z, Liu H, Nix J, Xu R, Knoverek CR, Bowman GR, Amarasinghe GK, Sibley LD. The intrinsically disordered protein TgIST from Toxoplasma gondii inhibits STAT1 signaling by blocking cofactor recruitment. Nat Commun. 2022 Jul 13;13(1):4047. doi: 10.1038/s41467-022-31720-7. PMID: 35831295; PMCID: PMC9279507.

Radke JB, Melillo B, Mittal P, Sharma M, Sharma A, Fu Y, Uddin T, Gonse A, Comer E, Schreiber SL, Gupta AK, Chatterjee AK, Sibley LD. Bicyclic azetidines target acute and chronic stages of Toxoplasma gondii by inhibiting parasite phenylalanyl t-RNA synthetase. Nat Commun. 2022 Jan 24;13(1):459. doi: 10.1038/s41467-022-28108-y. PMID: 35075105; PMCID: PMC8786932.

Rinkenberger N, Abrams ME, Matta SK, Schoggins JW, Alto NM, Sibley LD. Overexpression screen of interferon-stimulated genes identifies RARRES3 as a restrictor of Toxoplasma gondii infection. Elife. 2021 Dec 6;10:e73137. doi: 10.7554/eLife.73137. PMID: 34871166; PMCID: PMC8789288.

Fu Y, Brown KM, Jones NG, Moreno SN, Sibley LD. Toxoplasma bradyzoites exhibit physiological plasticity of calcium and energy stores controlling motility and egress. Elife. 2021 Dec 3;10:e73011. doi: 10.7554/eLife.73011. PMID: 34860156; PMCID: PMC8683080.

Saraav I, Cervantes-Barragan L, Olias P, Fu Y, Wang Q, Wang L, Wang Y, Mack M, Baldridge MT, Stappenbeck T, Colonna M, Sibley LD. Chronic Toxoplasma gondii infection enhances susceptibility to colitis. Proc Natl Acad Sci U S A. 2021 Sep 7;118(36):e2106730118. doi: 10.1073/pnas.2106730118. PMID: 34462359; PMCID: PMC8433586.

Rosenberg A, Sibley LD. Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosis. Cell Host Microbe. 2021 Jul 14;29(7):1186-1198.e8. doi: 10.1016/j.chom.2021.04.016. Epub 2021 May 26. PMID: 34043960; PMCID: PMC8711274.

Matta SK, Rinkenberger N, Dunay IR, Sibley LD. Toxoplasma gondii infection and its implications within the central nervous system. Nat Rev Microbiol. 2021 Jul;19(7):467-480. doi: 10.1038/s41579-021-00518-7. Epub 2021 Feb 24. PMID: 33627834.

Rosenberg A, Luth MR, Winzeler EA, Behnke M, Sibley LD. Evolution of resistance in vitro reveals mechanisms of artemisinin activity in Toxoplasma gondii. Proc Natl Acad Sci U S A. 2019 Dec 5;116(52):26881–91. doi: 10.1073/pnas.1914732116. Epub ahead of print. PMID: 31806760; PMCID: PMC6936365.

Janetka JW, Hopper AT, Yang Z, Barks J, Dhason MS, Wang Q, Sibley LD. Optimizing Pyrazolopyrimidine Inhibitors of Calcium Dependent Protein Kinase 1 for Treatment of Acute and Chronic Toxoplasmosis. J Med Chem. 2020 Jun 11;63(11):6144-6163. doi: 10.1021/acs.jmedchem.0c00419. Epub 2020 Jun 1. PMID: 32420739; PMCID: PMC7325724.

Matta SK, Olias P, Huang Z, Wang Q, Park E, Yokoyama WM, Sibley LD. Toxoplasma gondii effector TgIST blocks type I interferon signaling to promote infection. Proc Natl Acad Sci U S A. 2019 Aug 14. pii: 201904637. doi:10.1073/pnas.1904637116. PubMed PMID: 31413201.

Drewry LL, Jones NG, Wang Q, Onken MD, Miller MJ, Sibley LD. The secreted kinase ROP17 promotes Toxoplasma gondii dissemination by hijacking monocyte tissue migration. Nat Microbiol. 2019 Jul 22. doi: 10.1038/s41564-019-0504-8. PubMed PMID: 31332383.

Wilke G, Funkhouser-Jones LJ, Wang Y, Ravindran S, Wang Q, Beatty WL, Baldridge MT, VanDussen KL, Shen B, Kuhlenschmidt MS, Kuhlenschmidt TB, Witola WH, Stappenbeck TS, Sibley LD. A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability. Cell Host Microbe. 2019 Jul 10;26(1):123-134.e8. doi: 10.1016/j.chom.2019.05.007. PubMed PMID: 31231046; PubMed Central PMCID: PMC6617391.

Brown KM, Sibley LD. Essential cGMP Signaling in Toxoplasma Is Initiated by a Hybrid P-Type ATPase-Guanylate Cyclase. Cell Host Microbe. 2018 Dec 12;24(6):804-816.e6. doi: 10.1016/j.chom.2018.10.015. PubMed PMID: 30449726; PubMed Central PMCID: PMC6292738.

Long S, Anthony B, Drewry LL, Sibley LD. A conserved ankyrin repeat-containing protein regulates conoid stability, motility and cell invasion in Toxoplasma gondii. Nat Commun. 2017 Dec 21;8(1):2236. doi: 10.1038/s41467-017-02341-2.PubMed PMID: 29269729; PubMed Central PMCID: PMC5740107.

Rutaganira FU, Barks J, Dhason MS, Wang Q, Lopez MS, Long S, Radke JB, Jones NG, Maddirala AR, Janetka JW, El Bakkouri M, Hui R, Shokat KM, Sibley LD. Inhibition of Calcium Dependent Protein Kinase 1 (CDPK1) by Pyrazolopyrimidine Analogs Decreases Establishment and Reoccurrence of Central Nervous System Disease by Toxoplasma gondii. J Med Chem. 2017 Dec 28;60(24):9976-9989. doi:10.1021/acs.jmedchem.7b01192. PubMed PMID: 28933846; PubMed Central PMCID: PMC5746462.

Hakimi MA, Olias P, Sibley LD. Toxoplasma Effectors Targeting Host Signaling and Transcription. Clin Microbiol Rev. 2017 Jul;30(3):615-645. PMID: 28404792.

Olias P, Etheridge RD, Zhang Y, Holtzman MJ, Sibley LD. Toxoplasma Effector Recruits the Mi-2/NuRD Complex to Repress STAT1 Transcription and Block IFN-γ-Dependent Gene Expression. Cell Host Microbe. 2016 Jul 13;20(1):72-82.PubMed PMID: 27414498.

Behnke MS, Dubey JP, Sibley LD. Genetic Mapping of Pathogenesis Determinants in Toxoplasma gondii. Annu Rev Microbiol. 2016 Sep 8;70:63-81. PubMed PMID: 27359216.

Lorenzi H, Khan A, Behnke MS, Namasivayam S, Swapna LS, Hadjithomas M, Karamycheva S, Pinney D, Brunk BP, Ajioka JW, Ajzenberg D, Boothroyd JC, Boyle JP, Dardé ML, Diaz-Miranda MA, Dubey JP, Fritz HM, Gennari SM, Gregory BD, Kim K, Saeij JP, Su C, White MW, Zhu XQ, Howe DK, Rosenthal BM, Grigg ME, Parkinson J, Liu L, Kissinger JC, Roos DS, Sibley LD. Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes. Nat Commun. 2016 Jan 7;7:10147. PubMed PMID: 26081631.

Selleck EM, Orchard RC, Lassen KG, Beatty WL, Xavier RJ, Levine B, Virgin HW, Sibley LD. A Noncanonical Autophagy Pathway Restricts Toxoplasma gondii Growth in a Strain-Specific Manner in IFN-γ-Activated Human Cells. MBio. 2015 Sep 8;6(5):e01157-15. PubMed PMID: 26081631.

Drewry LL, Sibley LD. Toxoplasma Actin Is Required for Efficient Host Cell Invasion. MBio. 2015 Jun 16;6(3):e00557. PubMed PMID: 26081631.

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